streak plate method lab report


To attempt to grow individual colonies from a source plate. MIC101 Lab Report Grading Rubric 20points MIC101 Lab Report.


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LAB REPORT 2 TITLE.

. Identify shapes of bacterial shapes. Streak Plate Use sterile loop to collect sample from. Plate streak the mixed culture solely in one direction across the entire plate in one long motion.

Show the loop to flame and then let it cool downThen go to the edge of each line of the first quadrant and extend this to second quadrants sets1 Again sterilize the loop and in the same manner streak the third set of quadrants. The first lab period of this experiment an unknown was assigned. Kitchen Streak Plate and Lab Report Chasatie Wisdom BS203OL11808 Introduction In this experiment I am trying to procure a pure sample of my microbe.

Produce a streak plate with isolated colonies and to determine the correct Gram stain colour shape and arrangement of 10 microorganisms. The dilution or isolation by streaking procedure was originally developed by Loeffler and Gaffky in Kochs laboratory which involves the dilution of bacteria by systematically streaking. The inoculum is streaked over the agar surface to isolate colonies on at least a portion of the plate.

Initial Streaking of Unknown in First lab Period. View Lab Report - Micro Lab- Lab Report Streak Plate Method from BIO 113 at Winston-Salem State University. The modern streak plate method has evolved from the efforts by Robert Koch and other microbiologists to obtain pure culture of bacteria in order to study them.

The plate was incubated at 37 degrees Celsius until the following lab period. We took universal containers 20cm 3 of nutrient agar melted at. Source Plate Sterile loop Sterile Plate Petri Dish Bottle of Sterile Agar Pipette Bottle of Culture Microscope Slides METHODS.

Introduction Hypothesis Methods Results Conclusion Resources Writing Tips. You should end up with isolated colonies somewhere in your last streak. Lab Reports and Scientific Papers.

For the other TSA plate using the above procedure as a guide perform the streak plate using the four-phase method. For Exercise 1-5 you may be working as a team to be preparing streak plates and will observe the professor perform a pour plate method for isolating colonies. Tape the plate closed and incubates the plate in an inverted position in an incubator for 24-48 hours.

While doing the isolation method is to extract the bacteria that are combined to see if the germs decrease while being tested. The plates were cultured using the streak plate technique. Singh 20 September 2016 Streak Plate Method of.

In Streak plate technique a sterilized. A common method for the isolation of a pure culture from a mixture is by streaking plates. This lab report has 2 parts.

Area of the second streak from the area 1 yields gives dense growth. The streak plate method is a rapid qualitative isolation method for obtaining discrete colonies from a mixed population. Randi Blocker MIC 101 Lab Report 3 Isolation Methods Quadrant Streak Plate Method Introduction.

Samplepractice exam 9 June 2017 answers. Ideal results require that only a small amount of inoculum be used and that the surface of the plate be free of droplets of condensed. Show the inoculating loop to the flame and keep it.

Flame the loop before putting it aside. To achieve this I will utilize the quadrant streak. Zharia Ward BIO 113-03 Dr.

Your report must include the following in 1-2 pages. Area of initial inoculation and the first streak yields heavy growth. Incubate all plates at 35-37C for 36-48 hours.

The unknown that corresponds to this lab report is unknown 19. Streak and Pour Plate TechniquesBacterial Shapes PURPOSE. Repeat the technique for the fourth set of quadrants.

Area of initial inoculation and the first streak yields heavy growth. 2 Write your lab report in essay format. Calcium Carbonate Lab report.

It was originally developed by the two bacteriologists Loeffler and Gaffkey in the lab of Robert Koch Father of Bacteriology. The main difference shall be how far the plate is turned during each streak. The unknown was cultured during the first lab experiment using TSA blood agar MacConkey as the media.

How to Write your Lab Report. You are here-home-Biotechnology and Biomedical Engineering-Microbiology Virtual Lab I-Streak Plate Method. When turning in the lab make sure you include information relevant for BOTH parts.

Complete the virtual lab in MH Connect. Characteristics done as a demonstration and streak plating etc. Quadrant streaks are the best with isolating pure colonies because you separate microbes from a dense population to a smaller and smaller population until you have a pure.

Tape the plate closed and incubates the plate in an inverted position in an incubator for 24-48 hours. The Streak Plate technique for the isolation of microorganism is the most practical method of obtaining discrete and well-developed colonies of the microbe in pure cultures. First we prepared an agar plate.

Staphylococcus aureus colony on nutrient agar. Concentration of the bacteria is low so when there is a single bacterium or a group they surface as a colony on the surface of the agar plate.


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